multiinter — bedtools 2.31.0 documentation

# multiinter

`bedtools multiinter` identifies common intervals among multiple (and subsets thereof)

  sorted BED/GFF/VCF files.

Note

1. All files must be sorted in the same mmanner (e.g., `sort -k 1,1 -k2,2n in.bed > in.sorted.bed`)

## Usage and option summary

Usage:

    bedtools multiinter [OPTIONS] -i FILE1 FILE2 .. FILEn

(or):

    multiIntersect [OPTIONS]  -i FILE1 FILE2 .. FILEn

Option | Description
--- | ---
-header | Print a header line (chrom/start/end + names of each file).
-names | A list of names (one/file) to describe each file in -i. These names will be printed in the header line.
-g | Use genome file to calculate empty regions.
-empty | Report empty regions (i.e., start/end intervals w/o | values in all files). Requires the ‘-g FILE’ parameter.
-filler TEXT | Use TEXT when representing intervals having no value. | Default is ‘0’, but you can use ‘N/A’ or any text.
-examples | Show usage examples on the command line.

## Default behavior

By default, `bedtools multiinter` will inspect all of the intervals in each input file and report the sub-intervals that are overlapped by 0, 1, 2, … N files. The default output format is as follows:

1. chromosome (or entire genome)

2. 0-based start coordinate of the sub-interval.

3. 1-based end coordinate of the sub-interval.

4. The number of files whose intervals overlap this sub interval at least once.

5. The list of file numbers (by order on the command line) whose intervals overlap this sub interval at least once.

6. Columns reflecting whether each file had (1) or did not have (0) 1 or more intervals overlapping this sub interval.

For example:

    $ cat a.bed
    chr1  6   12
    chr1  10  20
    chr1  22  27
    chr1  24  30

    cat b.bed
    chr1  12  32
    chr1  14  30

    $ cat c.bed
    chr1  8   15
    chr1  10  14
    chr1  32  34

    $ cat sizes.txt
    chr1  5000

    $ bedtools multiinter -i a.bed b.bed c.bed
    chr1        6       8       1       1       1       0       0
    chr1        8       12      2       1,3     1       0       1
    chr1        12      15      3       1,2,3   1       1       1
    chr1        15      20      2       1,2     1       1       0
    chr1        20      22      1       2       0       1       0
    chr1        22      30      2       1,2     1       1       0
    chr1        30      32      1       2       0       1       0
    chr1        32      34      1       3       0       0       1

## `-header` Add a header with columns names

For example:

    $ bedtools multiinter -header -i a.bed b.bed c.bed
    chrom       start   end     num     list    a.bed   b.bed   c.bed
    chr1        6       8       1       1       1       0       0
    chr1        8       12      2       1,3     1       0       1
    chr1        12      15      3       1,2,3   1       1       1
    chr1        15      20      2       1,2     1       1       0
    chr1        20      22      1       2       0       1       0
    chr1        22      30      2       1,2     1       1       0
    chr1        30      32      1       2       0       1       0
    chr1        32      34      1       3       0       0       1

## `-names` Add custom labels for each file in the header

For example:

    $ bedtools multiinter -header -names A B C -i a.bed b.bed c.bed
    chrom       start   end     num     list    A       B       C
    chr1        6       8       1       1       1       0       0
    chr1        8       12      2       1,3     1       0       1
    chr1        12      15      3       1,2,3   1       1       1
    chr1        15      20      2       1,2     1       1       0
    chr1        20      22      1       2       0       1       0
    chr1        22      30      2       1,2     1       1       0
    chr1        30      32      1       2       0       1       0
    chr1        32      34      1       3       0       0       1

## `-empty` Report the sub intervals not covered by any file

Note that this option requires a `-g` file so that it knows the full range of each chromosome or contig.

For example:

     $ bedtools multiinter -header -names A B C -i a.bed b.bed c.bed -empty -g sizes.txt
    chrom  start   end     num     list    A       B       C
    chr1   0       6       0       none    0       0       0
    chr1   6       8       1       A       1       0       0
    chr1   8       12      2       A,C     1       0       1
    chr1   12      15      3       A,B,C   1       1       1
    chr1   15      20      2       A,B     1       1       0
    chr1   20      22      1       B       0       1       0
    chr1   22      30      2       A,B     1       1       0
    chr1   30      32      1       B       0       1       0
    chr1   32      34      1       C       0       0       1
    chr1   34      5000    0       none    0       0       0

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